Journal
STRUCTURE
Volume 13, Issue 12, Pages 1765-1773Publisher
CELL PRESS
DOI: 10.1016/j.str.2005.08.016
Keywords
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Funding
- NIGMS NIH HHS [P01 GM66521] Funding Source: Medline
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The E coli pyruvate and 2-oxoglutarate dehydrogenases are two closely related, large complexes that exemplify a growing number of multiprotein machines whose domains have been studied extensively and modeled in atomic detail, but whose quaternary structures have remained unclear for lack of an effective imaging technology. Here, electron cryotomography was used to show that the E1 and E3 subunits of these complexes are flexibly tethered similar to 11 nm away from the E2 core. This result demonstrates unambiguously that electron cryotomography can reveal the relative positions of features as small as 80 kDa in individual complexes, elucidating quaternary structure and conformational flexibility.
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