Journal
PROTOPLASMA
Volume 226, Issue 3-4, Pages 217-221Publisher
SPRINGER WIEN
DOI: 10.1007/s00709-005-0096-4
Keywords
protoplast; thin-alginate-layer technique; extra-thin-alginate-film technique; Nicotiana tabacum; Lotus corniculatus
Categories
Ask authors/readers for more resources
This paper reports an efficient protoplast culture technique, the extra thin alginate film technique. The development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. The efficiency of this technique was evaluated with two model plant systems, viz., Nicotiana tabacum and Lotus corniculatus, and a comparison was made with the thin alginate layer technique, another efficient protoplast culture system. Results indicate that the culture technique with extra thin alginate film is as efficient as the technique with thin alginate layer, with many additional advantages. The present innovation overcomes most of the limitations of protoplast culture techniques described so far and can now be applied to a wide variety of crops to check its general applicability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available