Journal
JOURNAL OF VIROLOGY
Volume 79, Issue 24, Pages 15567-15572Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.79.24.15567-15572.2005
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Funding
- NIAID NIH HHS [R01AI6400] Funding Source: Medline
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TRIM-CypA is an owl monkey-specific variant of the retrovirus restriction factor TRIM5 alpha. Here, we exploit its modular domain organization and cyclosporine sensitivity to probe the kinetics and mechanism of TRIM5-mediated restriction. Time of addition/withdrawal experiments reveal that inhibition of incoming human immunodeficiency virus type 1 capsids by TRIM-CypA occurs within minutes of their delivery to the target cell cytoplasm. However, while TRIM-CypA restriction is partly dependent on a RING domain, restriction occurs independently of the ubiquitin/proteasome system. Moreover, tagged TRIM-CypA proteins can be fully active as restriction factors without forming cytoplasmic bodies.
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