Journal
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
Volume 27, Issue 12, Pages 1061-1067Publisher
WILEY
DOI: 10.1002/hed.20288
Keywords
DNA; methylation; oral cancer
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Funding
- NCI NIH HHS [R03 CA 91273, R03 CA091273] Funding Source: Medline
- NIDCR NIH HHS [U54 DE014257] Funding Source: Medline
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Background. Although alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking-related cancers of the head and neck. Methods. We evaluated the status of GDM by using monoclonal antibodies specific for 5-methylcytosine (5-mc) in oral squamous cell carcinoma (SCC) specimens of 48 cigarette smokers who had SCC develop and in 93 age-, race-, and sex-matched smokers who did not. Results. Percentages of cells positive for 5-mc immuno-staining of DNA of SCC and dysplastic lesions were significantly higher than those of normal oral epithelial cells from cancer subjects and from noncancer subjects. The degree of DNA methylation was unrelated to DNA content. Conclusions. The pattern of GDM in oral SCCs is different from that of lung SCCs. The differences in nutrient risk factor profiles that are related to GDM and differential activity of DNA methyltranferases between oral and lung SCCs may explain these observations. (c) 2005 Wiley Periodicals, Inc.
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