Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 75A, Issue 3, Pages 588-594Publisher
WILEY
DOI: 10.1002/jbm.a.30459
Keywords
ceramic scaffolds; osteoblasts; cell adhesion; cell differentiation
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The objective of this study was to compare adhesion, replication, and differentiation of human osteoblasts (OPC1 cell line) on two different ceramic substrates: a bioinert alumina (Al2O3), and a bioactive hydroxyapatite (HAp). Scanning electron microscopy (SEM) and confocal scanning microscopy were used to assess production and distribution of specific osteoblast proteins. Cells grew readily on HAp and alumina. On alumina and HAp, OPC1 cells produced distinctive cell-to-cell and cell-to-scaffold filopodia and microextensions. OPC1 cells proliferated faster on HAp than on alumina substrates. Adhesion was further assessed by observing production of the protein vinculin. On alumina, vinculin was detected throughout the cytoplasm, with some peripheral punctate regions. On HAp, OPC1 cells developed few punctate peripheral regions of vinculin; rather, the protein was strongly localized within the cytoplasm. Finally, we measured accumulation of the marker osteopontin (OPN). OPN was first detectable on day 5 and increased through day 11 of culture on alumina. OPC1 cells on HAp produced substantial amounts of OPN beginning at day 5, with apparent secretion from the cells by culture day 11. These results suggest that the function of osteoblasts is strongly affected by the properties of ceramic surfaces, and rapid attachment of osteoblasts may result in earlier differentiation on HAp than on alumina. (c) 2005 Wiley Periodicals, Inc.
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