4.4 Article

Cellular and subcellular localization of kainic acid in the marine red alga Digenea simplex

Journal

CELL AND TISSUE RESEARCH
Volume 322, Issue 3, Pages 491-502

Publisher

SPRINGER
DOI: 10.1007/s00441-005-0035-x

Keywords

kainic acid; red algae; immunolabeling; pit plug; Digenea simplex (Ceramiales, Rhodomelaceae)

Categories

Ask authors/readers for more resources

Polyclonal antibodies specific for the excitatory amino acid, kainic acid (KA), were raised in rabbits. The antibody recognized KA but did not cross-react with other structurally related amino acids, including glutamate. We used this anti-KA antibody to localize KA immunohistochemically in the KA-producing red alga Digenea simplex. KA immunoreactivity was most dense in the fine cylindrical thallus, which covers the middle to upper part of the alga. The cortical cells, but not the inner layers of the main axis, and cells of the rhizoid were also stained with this antibody. The presence of KA in cells that cover the surface of the alga might reflect its role in chemical defense. At the subcellular level, KA immunoreactivity was most intense in the nucleus, pit plugs, and the electron-dense areas denoted as granule bodies, which were found only in the pericentral cells of the thallus.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available