Journal
JOURNAL OF DENTAL RESEARCH
Volume 84, Issue 12, Pages 1165-1171Publisher
INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R
DOI: 10.1177/154405910508401214
Keywords
Streptococcus; Fusobacterium nucleatum; Prevotella intermedia; Campylobacter; Eikenella corrodens; Treponema denticola; Gemella haemolysans; Granulicatella adiacens; buccal cells; bacterial invasion
Categories
Funding
- NIDCR NIH HHS [DE 14214] Funding Source: Medline
Ask authors/readers for more resources
Previously, we reported that intracellular Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. In this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify Fusobacterium nucleatum, Prevotella intermedia, oral Campylobacter species, Eikenella corrodens, Treponema denticola, Gemella haemolysans, Granulicatella adiacens, and total streptococci within buccal epithelial cells. All those species were found within buccal cells. In every case, species recognized by green- labeled species- specific probes were accompanied by other bacteria recognized only by a red- labeled universal probe. Streptococci appeared to be a major component of the polymicrobial intracellular flora, being present at a level from one to two logs greater than the next most common species ( G. adiacens). This is similar to what is observed in oral biofilms, where diverse species interact in complex communities that often are dominated by streptococci.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available