Journal
BIOMATERIALS
Volume 26, Issue 36, Pages 7636-7641Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2005.05.001
Keywords
hydroxyapatite.; cell printing; osteoblast
Funding
- NHLBI NIH HHS [R01-HL60435] Funding Source: Medline
- NIGMS NIH HHS [GM067445, GM065364] Funding Source: Medline
Ask authors/readers for more resources
This paper describes simple, inexpensive, and potentially generic methodology for generating patterns of mammalian cells on porous scaffolds for tissue engineering using replica printing. Circular patterns (diameter: 200, 700, and 1000 mu m) of human osteoblasts were transferred directly from topographically patterned agarose stamps onto porous hydroxyapatite scaffolds or onto fibronectin-coated glass slides. The use of hydrogel stamps provided a wet, biocompatible surface and maintained the viability of cells adsorbed on stamps during the patterning process. stamps inked once with suspensions of cells allowed the repeated patterning of substrates. Direct stamping of human osteoblasts (and, potentially other mammalian cells) can be used to control the size, spacing, and geometry of patterns of cells printed on porous tissue engineering substrates. This approach may find use in controlling the spatial invasion of scaffolds, promoting the hierarchical organization of cells, and in controlling cell-cell interactions as a step in preservation of phenotypes of cells. (c) 2005 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available