Journal
CELL STRUCTURE AND FUNCTION
Volume 30, Issue 1-2, Pages 1-6Publisher
JAPAN SOC CELL BIOLOGY
DOI: 10.1247/csf.30.1
Keywords
vitamin D-3; differetitiation; gene expression; initiation factor; HL-60
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The physiologically active metabolite of vitamin D-3, 1 alpha,25-dihydroxyvitamin D-3 (DVD), is a potent inducer of cell differentiation in human myeloid leukemia cells. In the present study, we examined changes in gene expression during DVD-induced cell differentiation of promyelocytic HL-60 cells employing a DNA microarray technique. The results identified 7 up-regulated and 9 down-regulated genes with a change greater than 1.5-fold after the DVD-treatment for both 2 and 6 days. Seven of these genes were further examined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that findings obtained from the DNA microarray analysis and RT-PCR are generally comparable with each other. Gene expression of the subunits of eukaryotic translation initiation factor 2 was then examined by methods including RT-PCR and real-time PCR. The results indicated the suppression of these genes, suggesting a linkage to differentiation-associated growth inhibition of these cells.
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