Journal
JAPANESE JOURNAL OF PHYSIOLOGY
Volume 55, Issue 6, Pages 379-383Publisher
CENTER ACADEMIC PUBL JAPAN
DOI: 10.2170/jjphysiol.S655
Keywords
Cl- channel; ClC-3; cardiomyocytes
Categories
Ask authors/readers for more resources
It is controversial whether the CIC-3 protein, which is one of the voltage-dependent chloride channel CIC family members, is a candidate for the volume-sensitive outwardly rectifying (VSOR) Cl- channel per se or its regulator. Here, for the first time, we examined the single-channel properties of the VSOR Cl- channel in ventricular myocytes isolated from CIC-3-deficient mice. The single-channel current induced by cell swelling exhibited Cl- selectivity, mild outward rectification, and an intermediate unitary conductance (around 38 pS). A Cl- channel blocker, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), reversibly inhibited the outward current. These single-channel properties were identical with those in CIC-3 expressing wild-type ventricular myocytes. These results indicate that the single-channel activity of the VSOR Cl- channel is independent of the expression of CIC-3 proteins in mouse ventricular myocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available