Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 35, Issue 12, Pages 3433-3441Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/eji.200535364
Keywords
human B cell subsets; ABC transporters; B cell turnover
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Funding
- NIAID NIH HHS [U19AI057266/01] Funding Source: Medline
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The exact identification of B cell subsets is instrumental to understand their dynamics under physiological and pathological conditions. Human memory B cells are currently identified according to the expression of CD27, which is absent on naive B cells. We report here that the ATP-binding cassette (ABC)B1 transporter is exclusively present on mature CD27(-) naive B cells, while it is absent in CD27(+) memory B cells and in a heterogeneous subset of CD27- cells that comprise both switch memory and transitional B cells. Thus, ABCB1 activity precisely discriminates naive from transitional and all memory B cells. Using this improved method to discriminate human B cell subsets, and Ki67 staining to identify recently divided cells, we show that in both cord blood and adult peripheral blood, mature naive B cells are quiescent while transitional B cells and memory B cells have a high in vivo turnover.
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