Journal
JOURNAL OF IMMUNOLOGY
Volume 175, Issue 11, Pages 7457-7465Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.175.11.7457
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Funding
- Medical Research Council [G9800943, G9202171] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- MRC [G9202171, G9800943] Funding Source: UKRI
- Medical Research Council [G9202171, G9800943] Funding Source: researchfish
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Clinical and low passage strains of human CMV (HCMV) encode an additional MHC class I-related molecule UL142, in addition to the previously described UL18. The UL142 open reading frame is encoded within the ULb' region which is missing from a number of common high passage laboratory strains. Cells expressing UL142 following transfection, and fibroblasts infected with a recombinant adenovirus-expressing UL142, were used to screen both polyclonal NK cells and NK cell clones, in a completely autologous system. Analysis of 100 NK cell clones derived from five donors, revealed 23 clones that were inhibited by fibroblasts expressing UL142 alone. Small-interfering RNA-mediated knockdown, of UL142 mRNA expression in HCMV-infected cells resulted in increased sensitivity to lysis. From these data we conclude that UL142 is a novel HCMV-encoded MHC class I-related molecule which inhibits NK cell killing in a clonally dependent manner.
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