Journal
JOURNAL OF MEDICAL MICROBIOLOGY
Volume 54, Issue 12, Pages 1183-1187Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.46160-0
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A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX.M) genes with 100% accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.
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