Journal
AVIAN DISEASES
Volume 49, Issue 4, Pages 488-493Publisher
AMER ASSOC AVIAN PATHOLOGISTS
DOI: 10.1637/7321-010405R1.1
Keywords
avian influenza virus (AIV); nonstructural protein (NSI); antibody; ELISA
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To differentiate avian influenza virus (AIV)-infected chickens vs. chickens immunized with inactivated avian influenza virus, an enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant nonstructural protein (NS1) as the diagnostic antigen, which was cloned from an AIV H9N2 subtype strain isolated during the avian influenza outbreak of 2003-04 and expressed in Escherichia coli. Antibodies to the AIV NSI protein was only detected in the sera of chickens experimentally infected with AIV but not in the sera of chickens immunized with inactivated vaccine. This ELISA is useful for serological diagnosis to distinguish chickens infected with influenza viruses from those immunized with inactivated vaccine.
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