Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 71, Issue 12, Pages 8958-8962Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.71.12.8958-8962.2005
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Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.
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