Journal
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
Volume 193, Issue 6, Pages 1966-1971Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.ajog.2005.04.052
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Objective: Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status. Study design: Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of RHD. The presence of fetal DNA was confirmed by detection of SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat. Results: Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3 RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls. Conclusion: Fetal RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases. (c) 2005 Mosby, Inc. All rights reserved.
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