Journal
FEBS LETTERS
Volume 579, Issue 29, Pages 6653-6658Publisher
WILEY
DOI: 10.1016/j.febslet.2005.09.104
Keywords
IL-4; Stat6; mast cells; fibroblasts; co-culture; histamine; SCF
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IL4 plays a crucial role in the pathogenesis of allergic diseases, such as the induction of IgE synthesis and the development of mast cells. To further understand the effect of IL-4 on mast cells in skin, we utilized a mast cell/fibroblast co-culture system as an in vitro model of dermal mast cells. IL-4 induced mast cell growth in the culture with fibroblasts. Immunoblot analysis revealed that IL-4 activated Stat6 in both mast cells and fibroblasts. The over-expression of dominant-negative Stat6 in fibroblasts in the presence of IL-4 decreased the histamine content per mast cell, but not the number of mast cells. In contrast, the over-expression of constitutively-active Stat6 in fibroblasts increased the histamine content per mast cell, indicating that the activation of Stat6 in fibroblasts supports the maturation of mast cells co-cultured with fibroblasts. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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