Journal
MOLECULAR CELL
Volume 20, Issue 5, Pages 793-799Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2005.10.016
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Chicken B lymphocyte precursors and DT40 cells diversify their immunoglobulin-variable (IgV) genes through homologous recombination (HR)-mediated Ig gene conversion. To identify DNA polymerases that are involved in Ig gene conversion, we created DT40 clones deficient in DNA polymerase eta (pol eta), which, in humans, is defective in the variant form of xeroderma pigmentosum (XP-V). Pol eta is an error-prone translesion DNA synthesis polymerase that can bypass UV damage-induced lesions and is involved in IgV hypermutation. Like XP-V cells, pol eta-disrupted (pol eta) clones exhibited hypersensitivity to UV. Remarkably, pol eta cells showed a significant decrease in the frequency of both Ig gene conversion and double-strand break-induced HR when compared to wild-type cells, and these defects were reversed by complementation with human pol eta. Our findings identify a DNA polymerase that carries out DNA synthesis for physiological HR and provides evidence that a single DNA polymerase can play multiple cellular roles.
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