Journal
EXPERIMENTAL CELL RESEARCH
Volume 311, Issue 2, Pages 251-264Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2005.09.005
Keywords
estrogen receptor; steroid receptors; development; embryonic stem cells; nuclear receptors; differentiation
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Funding
- NIDDK NIH HHS [R01 DK064243, R01 DK064243-01] Funding Source: Medline
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The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ER alpha, ER beta) are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen-dependent manner by endogenous ERs or by transiently overexpressed ER alpha. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation. (c) 2005 Elsevier Inc. All rights reserved.
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