Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 15, Issue 24, Pages 5459-5462Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2005.08.088
Keywords
UDP-GalNAc; enzymatic synthesis
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UDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. Both enzymes have been prepared in one step from I L cultures of transformed Escherichia coli and I he UDP-GalNAc produced has been purified by a simple procedure. The method described is a rapid ;and efficient means to produce UDP-GalNAc as well as analogues like UDP-N-azidoacetylgalactosamine (UDP-GalNAz). (c) 2005 Elsevier Ltd. All rights reserved.
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