Journal
FEMS MICROBIOLOGY LETTERS
Volume 253, Issue 2, Pages 281-288Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2005.09.047
Keywords
filamentous fungus; gene deletion; biological function; morphology
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Hydrophobins are fungal self-assembling proteins. Here, the hydrophobin genes hfb1 and hfb2 were deleted in Trichoderma reesei and their biological roles studied. Our results suggest that HFBI has a role in hyphal development and HFBII in sporulation. Sporulating colonies of the Delta hfb2 strain were wettable and sporulation was only 50% of the parent strain. Colonies of Delta hfb1 showed wettable and fluffy phenotype. In shaken liquid cultures, the hyphae of Delta hfb1 were thinner and biomass formation was slower compared to the parent strain while in static liquid cultures no aerial hyphae were formed. Expressing the Schizophyllum commune hydrophobin SC3 in the Delta hfb1 strain restored the formation of aerial hyphae. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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