Journal
FEBS LETTERS
Volume 579, Issue 30, Pages 6721-6725Publisher
WILEY
DOI: 10.1016/j.febslet.2005.10.068
Keywords
ERK; 5-hydroxytryptamine; migration; Rho kinase; vascular endothelium
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The purpose of the present study was to investigate whether 5-hydroxytryptamine (5-HT, serotonin) affects migration of vascular endothelial cells. 5-HT significantly enhanced migration of human aortic endothelial cells (HAECs), and this enhancement was completely inhibited by GR 55562, a 5-HT, receptor antagonist, and fluoxetine, a 5-HT transporter inhibitor, but was not affected by ketanserin, a 5-HT2 receptor antagonist. 5-HT stimulation increased RhoA and ERK activity of HAECs, and inhibitors of RhoA (Y-27632 and H-1152) and inhibitors of MEK (U0126 and PD98059) abolished the 5-HT-induced increase in migration velocity. Inhibition of Rho kinase by Y-27632 blocked stress fiber formation and rear release of HAECs. Thus, 5-HT has a potent enhancing action on migration of HAECs through activating the RhoA and ERK pathways following 5-HT, receptor stimulation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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