Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 102, Issue 51, Pages 18420-18424Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0507123102
Keywords
vesicular stomatitis virus; virus
Categories
Funding
- NIAID NIH HHS [R01 AI059371, AI059371, R01 AI059371-01A1, R37 AI059371] Funding Source: Medline
- NIGMS NIH HHS [R01 GM069891, R01 GM069891-04] Funding Source: Medline
Ask authors/readers for more resources
RNA interference (RNAi) is a sequence-specific gene-silencing mechanism triggered by exogenous dsRNA. In plants an RNAi-like mechanism defends against viruses, but the hypothesis that animals possess a similar natural antiviral mechanism related to RNAi remains relatively untested. To test whether genes needed for RNAi defend animal cells against virus infection, we infected wild-type and RNAi-defective cells of the nematode C elegans with vesicular stomatitis virus engineered to encode a GFP fusion protein. We show that upon infection, cells lacking components of the RNAi apparatus produce more GFP and infective particles than wild-type cells. Furthermore, we show that mutant cells with enhanced RNAi produce less GFP. Our observation that multiple genes required for RNAi are also required for resistance to vesicular stomatitis virus suggests that the RNAi machinery functions in resistance to viruses in nature.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available