Journal
CELL
Volume 123, Issue 7, Pages 1323-1335Publisher
CELL PRESS
DOI: 10.1016/j.cell.2005.09.043
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Cortical polarity regulates cell division, migration, and differentiation. Microtubules induce cortical polarity in yeast, but few examples are known in metazoans. We show that astral microtubules, kinesin Khc-73, and Discs large (Dig) induce cortical polarization of Pins/G alpha i in Drosophila neuroblasts; this cortical domain is functional for generating spindle asymmetry, daughtercell-size asymmetry, and distinct sibling fates. Khc-73 localizes to astral microtubule plus ends, and Dlg/Khc-73 and Dig/Pins co-immunoprecipitate, suggesting that microtubules induce Pins/G alpha i cortical polarity through Dlg/Khc-73 interactions. The microtubule/Khc-73/Dlg pathway acts in parallel to the well-characterized Inscuteable/ Par pathway, but each provides unique spatial and temporal information: The Inscuteable/Par pathway initiates at prophase to coordinate neuroblast cortical polarity with CNS tissue polarity, whereas the microtubule/Khc-73/Dlg pathway functions at metaphase to coordinate neuroblast cortical polarity with the mitotic spindle axis. These results identify a role for microtubules in polarizing the neuroblast cortex, a fundamental step for generating cell diversity through asymmetric cell division.
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