Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1101, Issue 1-2, Pages 122-135Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.09.080
Keywords
reversed-phase liquid chromatography; orthogonal method; pharmaceutical analysis
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A general procedure is proposed for the rapid development of a reversed-phase liquid chromatographic (RP-LC) separation that is orthogonal to a pre-existing (primary) method for the RP-LC separation of a given sample. The procedure involves a change of the mobile-phase organic solvent (B-solvent), the replacement of the primary column by one of very different selectivity, and (only if necessary) a change in mobile phase pH or the use of a third column. Following the selection of the orthogonal B-solvent, column and mobile phase pH, further optimization of peak spacing and resolution can be achieved by varying separation temperature and either isocratic %B or gradient time. The relative orthogonality of the primary and orthogonal RP-LC methods is then evaluated from plots of retention for one method versus the other. The present procedure was used to develop orthogonal methods for nine routine RP-LC methods from six pharmaceutical analysis laboratories. The relative success of this approach can be judged from the results reported here. (c) 2005 Elsevier B.V. All rights reserved.
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