Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1101, Issue 1-2, Pages 79-85Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2005.09.064
Keywords
macroporous monolithic gels; phage clones; lactoferrin; purification
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An affinity purification procedure for the direct purification of lactoferrin from defatted (skimmed) milk has been developed. The procedure is based on using selected phage clones expressing a peptide with high binding affinity for lactoferrin which were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 mu m) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 mu m) phage particles as ligands without any risk of blocking the monolithic column. Bound lactoferrin was eluted using 1 M NaC1 with a purity of > 95%. The technique presents a good alternative to conventional immunoaffinity chromatography for purification of a protein of interest from complex samples due to (i) the robustness of the system in terms of recovery and ligand leakage and (ii) economical aspect in terms of low ligand cost. (c) 2005 Elsevier B.V. All rights reserved.
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