Differential β-arrestin binding of AT1 and AT2 angiotensin receptors

Agonist stimulation of G protein-coupled receptors causes receptor activation, phosphorylation, P-arrestin binding and receptor internalization. Angiotensin II (AngII) causes rapid internalization of the AT, receptors, whereas AngII-bound AT(2) receptors do not internalize. Although the activation of the rat AT(1A) receptor with AngII causes translocation of beta-arrestin2 to the receptor, no association of this molecule with the AT(2) receptor can be detected after AngII treatment with confocal microscopy or bioluminescence resonance energy transfer. These data demonstrate that the two subtypes of angiotensin receptors have different mechanisms of regulation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.