Journal
FEBS LETTERS
Volume 580, Issue 1, Pages 345-350Publisher
WILEY
DOI: 10.1016/j.febslet.2005.12.025
Keywords
nucleophosmin; p53; stabilisation; interaction domains; chemical cross-linking; peptide analysis; SELDI
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We have used surface plasmon resonance to quantify the kinetics and stoichiometry of the interaction between p53 and nucleophosmin (NPM). Domains characterising the interface between the two proteins were identified by chemical cross-linking, proteolytic digestion and mass spectrometry based peptide mapping.We show that the C-terminal domain of NPM (residues 242-269) interacts with two regions of p53 (residues 175-196 and residues 343-363) which belong, respectively, to the DNA binding domain and the tetramerisation domain. Potential biological consequences of such interactions are discussed. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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