Journal
NATURE
Volume 439, Issue 7073, Pages 229-233Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature04343
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Funding
- Medical Research Council [G0200496] Funding Source: Medline
- Medical Research Council [G0200496] Funding Source: researchfish
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The Z-disk of striated and cardiac muscle sarcomeres is one of the most densely packed cellular structures in eukaryotic cells(1). It provides the architectural framework for assembling and anchoring the largest known muscle filament systems by an extensive network of protein - protein interactions, requiring an extraordinary level of mechanical stability. Here we show, using X-ray crystallography, how the amino terminus of the longest filament component, the giant muscle protein titin, is assembled into an antiparallel ( 2: 1) sandwich complex by the Z-disk ligand telethonin. The pseudosymmetric structure of telethonin mediates a unique palindromic arrangement of two titin filaments, a type of molecular assembly previously found only in protein DNA-complexes. We have confirmed its unique architecture in vivo by protein complementation assays, and in vitro by experiments using fluorescence resonance energy transfer. The model proposed may provide a molecular paradigm of how major sarcomeric filaments are crosslinked, anchored and aligned within complex cytoskeletal networks.
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