Journal
ANALYTICA CHIMICA ACTA
Volume 555, Issue 2, Pages 246-249Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2005.08.077
Keywords
microchip capillary electrophoresis; photon counter; fluorescence detection; fluorescence-5-isothiocyanate (FITC)
Categories
Ask authors/readers for more resources
A microchip capillary electrophoresis system with highly sensitive fluorescence detection is reported. The system was successfully constructed using an inverted fluorescence microscope, a highly sensitive photon counter, a photomultiplier tube (PNIT) and a capillary electrophotesis microchip. This system can be applied to the fluorescence detection with various wavelengths (300-600 nm). Different fluorescence reagents require different excitation wavelengths. The wavelengths of UV light (300-385 nm), blue light (450-480 nm) and green light (530-550 nm) are employed to excite Titan yellow, fluorescence-5-isothiocyanate (FITC) and Rhodamine 6G, respectively. The detection limit (S/N = 3) of FITC is 7 x 10(-10) M, which is 2-3 orders of magnitude lower than that obtained with the lamp-based fluorescence and PMT detection system and approaches the data gained by the laser-induced fluorescence detection. The linear relationship is excellent within the range of concentration 1.3 x 10(-9) to 6.5 x 10(-8) M FITC. It offers, a new method to widen the application of the lamp-based fluorescence detection. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available