Journal
ANALYTICA CHIMICA ACTA
Volume 555, Issue 2, Pages 242-245Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2005.09.005
Keywords
liquid chromatography; L-ascorbic acid; wine
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A rapid method for the identification and quantification of L-ascorbic acid in wines by direct injection liquid chromatography equipped with a UV detection was developed. The levels of ascorbic acid were determined using a polymeric PLRP-S 100 A (5 mu m) column (150 mm x 4.6 mm) with a mobile water/trifluoroacetic acid (99/1, v/v) phase. The method is rapid (less than 5 min) and sensitive (LOQ of 5 mg L(-1)). The calibration curve of ascorbic acid was linear (r = 0.999) over a concentration range between 1 and 200 mg L(-1). Repeatability was less than 2.5% and the recovery over 95%. (c) 2005 Elsevier B.V. All rights reserved.
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