Journal
ANALYTICA CHIMICA ACTA
Volume 555, Issue 2, Pages 292-298Publisher
ELSEVIER
DOI: 10.1016/j.aca.2005.09.007
Keywords
phosphate; microplate assay; luminescence; lanthanide
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A new luminescent europium probe is presented for the determination of phosphate (P) in microliter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 mu mol/L. The probe is excited at 400 nm and displays a large Stokes' shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 x 10(-6) mol/L to 7.5 x 10(-4) mol/L of phosphate, and the limit of detection is 3 mu mol/L. (c) 2005 Elsevier B.V. All rights reserved.
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