Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1102, Issue 1-2, Pages 294-301Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2005.10.057
Keywords
polymer monolith microextraction; poly(methacrylic acid-ethylene glycol dimethacrylate); monolithic capillary; capillary zone electrophoresis; angintensin II receptor antagonists
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Novel polymer monolith microextraction (PMME) using a poly(methacrylic acid-ethylene glycol dimethacrylate) (poly(MAA-EGDMA)) monolith in conjunction with capillary zone electrophoresis (CZE) was developed for the determination of several angiotensin II receptor antagonists (ARA-IIs) in human urine. The extraction device consisted of a regular plastic syringe (1 mL), a poly(MAA-EGDMA) monolithic capillary (2 cm x 530 mu m I.D.) and a plastic pinhead connecting the former two components seamlessly. The extraction was achieved by driving the sample solution through the monolithic capillary tube using a syringe infusion pump, and for the desorption step, an aliquot of organic solvent was injected via the monolithic capillary and collected into a vial for subsequent analysis by CZE. The best separation was realized at 25 kV using a buffer that consisted of 50% acetonitrile and 50%, buffer solution (v/v) containing 10 mM disodium hydrogenphosphate (adjusted to pH 2.3 with 1 M hydrochloric acid). The method was successfully applied to the determination of telmisartan (T), irbesartan (I) and losartan (L) in urine samples with candesartan (C) as internal standard, yielding the detection limit of 15-20 ng/mL. Close correlation coefficients (R > 0.999) and excellent method reproducibility were obtained for all the analytes over a linear range of 0.08-3 mu g/mL. (c) 2006 Elsevier B.V. All rights reserved.
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