Journal
GENES & DEVELOPMENT
Volume 20, Issue 2, Pages 153-158Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1382806
Keywords
alternative splicing; microarray analysis; nonsense-mediated mRNA decay; premature termination codon
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Funding
- NIGMS NIH HHS [R01 GM059614, GM059614] Funding Source: Medline
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Sequence-based analyses have predicted that similar to 35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants that are targeted by the process of nonsense-mediated mRNA decay (NMD). This led to speculation that AS may often regulate gene expression by activating NMD. Using AS microarrays, we show that PTC-containing splice variants are generally produced at uniformly low levels across diverse mammalian cells and tissues, independently of the action of NMD. Our results suggest that most PTC-introducing AS events are not under positive selection pressure and therefore may not contribute important functional roles.
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