Structural studies of the core region of Aeromonas salmonicida subsp salmonicida lipopolysaccharide

The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: [GRAPHICS] where R =alpha-D-Galp-(1 -> 4)-beta-D-GalpNAc-(1 -> or alpha-D-Galp-(1 -> (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida. Crown Copyright (c) 2005 Published by Elsevier Ltd. All rights reserved.