Determination of celecoxib in human plasma and breast milk by high-performance liquid chromatographic assay

A rapid and simple HPLC assay was developed for the determination of celecoxib in human plasma and breast milk. After proteins were precipitated with acetonitrile, celecoxib was resolved on a C18 column and detected by UV detection at 254 nm. Standard curves were linear over the concentration range 10-2000 mu g/L (r(2) > 0.99). Bias was <=+/- 15% from 20 to 2000 mu g/L in both matrices, intra- and inter-day coefficients of variation (imprecision) were < 10%, and the limit of quantification was 10 mu g/L. (c) 2005 Elsevier B.V. All rights reserved.