Journal
ANALYTICA CHIMICA ACTA
Volume 556, Issue 1, Pages 80-96Publisher
ELSEVIER
DOI: 10.1016/j.aca.2005.09.035
Keywords
polymer; microfabrication; DNA analysis; microfluidic; micro total analysis system
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The application of micro total analysis system (mu TAS) has grown exponentially in the past decade. DNA analysis is one of the primary applications of mu TAS technology. This review mainly focuses on the recent development of the polymeric microfluidic devices for DNA analysis. After a brief introduction of material characteristics of polymers, the various microfabrication methods are presented. The most recent developments and trends in the area of DNA analysis are then explored. We focus on the rapidly developing fields of cell sorting, cell lysis, DNA extraction and purification, polymerase chain reaction (PCR), DNA separation and detection. Lastly, commercially available polymer-based microdevices are included. (c) 2005 Elsevier B.V. All rights reserved.
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