Journal
SCIENCE
Volume 311, Issue 5759, Pages 392-394Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1123360
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Funding
- NHGRI NIH HHS [HG02238] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
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We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.
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