Absorption and metabolism of conjugated α-linolenic acid given as free fatty acids or triacylglycerols in rats

Background: Conjugated linoleic acid (CLA) is a group of polyunsaturated fatty acids which have been extensively studied in the past two decades. However, conjugated octadecatrienoic acid such as cis-9, trans-11, cis-15 and cis-9, trans-13, cis-15, recently identified, have not been extensively investigated. This work presents bioavailability and tissue incorporation of a mixture of conjugated octadecatrienoic (CLnA) acids ingested as free fatty acids (FFA) and triacylglycerols ( TAG). Results: Male Wistar rats were fed rumenic acid ( RA: cis-9, trans-11 18: 2) and a CLnA mixture (cis-9, trans-11, cis-15 18: 3 and cis-9, trans-13, cis-15 18: 3) as FFA and TAG for 8 days. RA and CLnA were both totally absorbed when given as FFA as well as TAG. Both isomers of CLnA as FFA or TAG were incorporated into neutral lipids. Metabolites up to 22: 6 conjugated isomers were present in liver and plasma phospholipids of rats fed the CLnA diets. Conclusion: Finally, CLnA are as well absorbed as RA in vivo and their incorporation into tissues and bioconversion are similar when ingested as FFA or as TAG.