Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1103, Issue 1, Pages 145-149Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.11.005
Keywords
biogenic amines; capillary zone electrophoresis; food; conductivity detection
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A capillary zone electrophoresis (CZE) method with conductometric detection of biogenic an-tines (cadaverine, putrescine, agmatine, histamine, tryptamine and tyramine) is described. The optimised background electrolyte was the following: 15 mM histidine + 5 mM adipic acid + 1.5 mM sulphuric acid + 0.1 mM ethylenediaminotetraacetic acid + 0.1% hydroxyethylcellulose + 50% methanol. A clear separation of six biogenic amines from other components of acidic sample extract was achieved within 10 min. Method characteristics, i.e., linearity (0-100 mu mol/ml), accuracy (recovery 86-107%), intra-assay repeatability (2-4%), and detection limit (2-5 mu mol/l) were evaluated. Low laboriousness, sufficient sensitivity, speed of analysis, and low running cost are important attributes of this method. The developed method was successfully applied on the determination of biogenic amines in selected food samples. (c) 2005 Elsevier B.V. All rights reserved.
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