Journal
OPTICS EXPRESS
Volume 14, Issue 2, Pages 759-766Publisher
OPTICAL SOC AMER
DOI: 10.1364/OPEX.14.000759
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We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy. (c) 2006 Optical Society of America.
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