Journal
FEBS LETTERS
Volume 580, Issue 2, Pages 677-681Publisher
WILEY
DOI: 10.1016/j.febslet.2005.12.063
Keywords
hydrogenase; metallocenter assembly; accessory protein interactions; nickel
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Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins. To analyze complex formation between these factors in Escherichia coli, they were genomically tagged and native multi-protein complexes were isolated. This method validated multiple interactions reported in separate studies from several organisms and defined a new complex containing the putative chaperone HybG and the large subunit of hydrogenase 1 or 2. The complex also includes HypE and HypD, which interact with each other before joining the larger complex. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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