Journal
CHEMICAL COMMUNICATIONS
Volume -, Issue 4, Pages 445-447Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b514103f
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The spore photoproduct lyase is a Fe-S/AdoMet DNA repair enzyme, which directly repairs spore lesions, induced by UV irradiation of spores, using an unknown radical mechanism. The air sensitive radical SAM enzyme was for the first time challenged with synthetically pure substrates. It was found that the enzyme recognizes a synthetic 5S-configured spore lesion without the central phosphodiester bond. The 5R-configured lesion is in contrast to current belief not a substrate.
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