Voltammetric determination of 6-benzylaminopurine (6-BAP) using an acetylene black-dihexadecyl hydrogen phosphate composite film coated glassy carbon electrode

An acetylene black-dihexadecyl hydrogen phosphate (denoted as AB-DHP) composite film coated glassy carbon electrode (GCE) is described for the determination of 6-benzylaminopurine (6-BAP). The electrochemical behavior of 6-BAP was investigated using cyclic voltammetry (CV), linear sweep voltammetry (LSV) and differential pulse voltammetry (DPV). Compared with the unmodified GCE, the AB-DHP film-modified GCE remarkably enhances the oxidation peak current of 6-BAP, suggesting great potential in the determination of trace level of 6-BAP. All the experimental parameters such as supporting electrolyte, the volume of modifier (AB-DHP suspension), scan rate, and accumulation conditions, were optimized. Finally, a sensitive and simple voltammetric method with a good linear relationship in the range of 2.0 x 10(-8) to 5.0 x 10(-6) mol/L, was developed for the determination of 6-BAP. The detection limit was also examined and a low value of 5.0 x 10(-9) mol/L for 5 min accumulation was obtained. This newly proposed method was successfully applied to detect 6-BAP in practical samples. (c) 2005 Elsevier B.V. All rights reserved.