Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 44, Issue 2, Pages 619-621Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.44.2.619-621.2006
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Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
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