Journal
CELLULOSE
Volume 13, Issue 1, Pages 63-71Publisher
SPRINGER
DOI: 10.1007/s10570-005-9031-2
Keywords
cellulose; glucuronan lyase; oligosaccharide; polyglucuronic acid; trichoderma
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The biodegradation of beta-(1,4)-polyglucuronic acids from cellulose acetate oxidation utilizing the TEMPO/NaBr/NaOCl system was investigated using a glucuronan lyase isolated from Trichoderma strain GL2. Products of the beta-elimination mechanism catalyzed by this enzyme were then purified by size-exclusion chromatography and characterized by ESI-Q/TOF-mass spectrometry and H-1-NMR spectroscopy. The analyses prove the complete biodegradation of this category of cellulose derivates and open the way to the large-scale production of oligouronides.
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