Journal
MOLECULAR AND CELLULAR PROBES
Volume 20, Issue 1, Pages 60-63Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2005.09.003
Keywords
DNA microarray; sensitivity; detection; Chlamydia trachomatis
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This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples. (c) 2005 Elsevier Ltd. All rights reserved.
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