Journal
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Volume 43, Issue -, Pages 65-70Publisher
WILEY
DOI: 10.1042/BA20050156
Keywords
anti-DNA autoantibody antigen; DNA damage; peroxynitrite; (ONOO-); peroxynitrite-modified DNA; systemic lupus erythematosus
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Native human DNA was modified by ONOO- (peroxynitrite), generated by the synergistic action of sodium nitroprusside [Na2Fe(CN)(5)NO], an NO donor, and pyrogallol, a superoxide donor. The modifications were analysed by UV absorption characteristics, fluorescence emission transitions, nuclease S1 digestibility and melting-temperature studies. Modified DNA was found to be highly immunogenic, inducing high-titre immunogen-specific antibodies in experimental animals. A maximum of 85% inhibition of the antibody binding was observed in competition ELISA with immunogen as inhibitor. SLE (systemic lupus erythematosus) anti-DNA autoantibodies recognized modified DNA as a better antigen than the native analogue. Modification of native DNA by ONOO-, forming neo-epitopes on the molecule, may be one of the factors for the induction of the autoimmune response seen in SLE.
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