Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 44, Issue 2-3, Pages 95-105Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2006.03.003
Keywords
actin microfilaments; cell division; endoplasmic reticulum; green fluorescent protein; microtubules; nuclear envelope; nuclear invaginations
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The endoplasmic reticulum (ER) of plant cells undergoes a drastic reorganization during cell division. In tobacco NT-1 cells that stably express a GFP construct targeted to the ER, we have mapped the reorganization of ER that occurs during mitosis and cytokinesis with confocal laser scanning microscopy. During division, the ER and nuclear envelope do not vesiculate. Instead, tubules of ER accumulate around the chromosomes after the nuclear envelope breaks down, with these tubules aligning parallel to the microtubules of the mitotic spindle. In cytokinesis, the phragmoplast is particularly rich in ER, and the transnuclear channels and imaginations present in many interphase cells appear to develop from ER tubules trapped in the developing phragmoplast. Drug studies. using oryzalin and latrunculin to disrupt the microtubules and actin microfilaments, respectively, demonstrate that during division, the arrangement of ER is controlled by microtubules and not by actin, which is the reverse of the situation in interphase cells. (c) 2006 Elsevier SAS. All rights reserved.
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