Journal
FEBS LETTERS
Volume 580, Issue 5, Pages 1263-1268Publisher
WILEY
DOI: 10.1016/j.febslet.2006.01.039
Keywords
SUN domain; nuclear envelope; lamin; inner nuclear membrane; UNC-84; SPAG4
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The SUN proteins are a conserved family of proteins in eukaryotes. Human UNC84A (Sun1) is a homolog of Caenorhabditis elegans UNC-84, a protein involved in nuclear anchorage and migration. We have analyzed targeting of UNC84A to the nuclear envelope (NE) and show that the N-terminal 300 amino acids are crucial for efficient NE localization of UNC84A whereas the conserved C-terminal SUN domain is not required. Furthermore, we demonstrate by combining RNA interference with immunofluorescence and fluorescence recovery after photobleaching analysis that localization and anchoring of UNC84A is not dependent on the lamin proteins, in contrast to what had been observed for C. elegans UNC-84. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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